Question 107325: I need some help figuring out how to do this. Do I make the measurement with a ruler? Directions: Using figure 7.1, measure the distance from the loading well to each band on your gel and set up a table with your results on the worksheet titled Assignment 7.3. Next, use algebra to determine the log of unknown fragments in proportion to a logarithmic scale. Then, using the graph on the worksheet titled Assignment 7.3, plot the points for each of the standards and draw a straight line that connects as many of these as possible. Use the X-axis to plot migration distance and the Y-axis to plot the log of the size or molecular weight. Now you can take the distance of migration for any unknown sample and find that point on the X-axis; then go across to the regression line; and from that point, go down to the Y-axis. This point will represent the log of the size of your unknown band.
Answer by scott8148(6628)   (Show Source):
You can put this solution on YOUR website! yes, measure with a ruler
this is some type of chromatography experiment
...the distance that the molecules diffuse is proportional to the log of their molecular weight
plotting the "standards" allows you to calibrate the experiment relative to the characteristics of the diffusion medium (gel)
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