Let the two perfect squares be
and 
Factor 53 to find possible values. Because 53 is prime, there are only two factors: 1 and 53.
x-y = 1
x+y = 53
Add both equations:
2x = 54
x = 27
This implies y = 26

Check:
729 - 676 = 53
Because we can only factor 53 into 1*53, there is only one pair of perfect squares that differ by 53.
For example, if we try
x - y = 53
x + y = 1
Adding gives:
2x = 54 ==> x = 27 and y = -26
This also works, but the perfect squares are the same as before: 729 and 676
Therefore, there is only one pair of perfect squares (729 and 676) whose difference is 53.
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If you try a different difference, say 
you will find that there are three sets of perfect squares that have a difference of 63 (64 and 1, 144 and 81, 1024 and 961) and these correspond to the factorizations of 63 (7 * 9, 3 * 21, and 1 * 63, respectively).